FIGURE

Fig. 5

ID

ZDB-FIG-160608-20

Publication

Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish

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Fig. 5

Cyp2aa9 regulate HSC development through the PGE2/cAMP/PKA signalling.

(a-f) Inhibition of PKA by H89 resulted in reduced cmyb expression in control, but did not exacerbate the HSC phenotype in cyp2aa9 morphants. Enhancement of cAMP by Forskolin increased the cmyb expression in control, and rescued the defective HSC development in cyp2aa9 morphants. (g-j) The inhibitory effects of H89 on HSC formation could not be rescued by the injection of cyp2aa9 mRNA. (k) In vivo PGE2 concentration measured by ELISA assay. n = 3 tubes of lysates, mean ± SD, ***p < 0.001, NS, not significant, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.

Expression Data

Gene:	myb
Fish:	WT WT + MO1-cyp2aa3,cyp2aa9
Condition:	chemical treatment by environment: forskolin
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Anatomical Term:	hematopoietic stem cell
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cyp2aa3,cyp2aa9
Condition:	chemical treatment by environment: forskolin
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Observed In:	hematopoietic stem cell hematopoietic stem cell differentiation prostaglandin-E synthase activity whole organism prostaglandin E2
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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