|ZFIN ID: ZDB-FIG-160608-20|
Cyp2aa9 regulate HSC development through the PGE2/cAMP/PKA signalling.
(a-f) Inhibition of PKA by H89 resulted in reduced cmyb expression in control, but did not exacerbate the HSC phenotype in cyp2aa9 morphants. Enhancement of cAMP by Forskolin increased the cmyb expression in control, and rescued the defective HSC development in cyp2aa9 morphants. (g-j) The inhibitory effects of H89 on HSC formation could not be rescued by the injection of cyp2aa9 mRNA. (k) In vivo PGE2 concentration measured by ELISA assay. n = 3 tubes of lysates, mean ± SD, ***p < 0.001, NS, not significant, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.
|myb||WT||standard conditions||Prim-25||hematopoietic stem cell||ISH|
|WT + MO1-cyp2aa3,cyp2aa9||standard conditions||Prim-25||hematopoietic stem cell||ISH|
|WT + MO1-cyp2aa3,cyp2aa9||chemical treatment by environment: forskolin||Prim-25||hematopoietic stem cell||ISH|
|Acknowledgments:||ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.|