ZFIN ID: ZDB-FIG-160608-20
Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish. Scientific Reports   6:26608 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 5

Cyp2aa9 regulate HSC development through the PGE2/cAMP/PKA signalling.

(a-f) Inhibition of PKA by H89 resulted in reduced cmyb expression in control, but did not exacerbate the HSC phenotype in cyp2aa9 morphants. Enhancement of cAMP by Forskolin increased the cmyb expression in control, and rescued the defective HSC development in cyp2aa9 morphants. (g-j) The inhibitory effects of H89 on HSC formation could not be rescued by the injection of cyp2aa9 mRNA. (k) In vivo PGE2 concentration measured by ELISA assay. n = 3 tubes of lysates, mean ± SD, ***p < 0.001, NS, not significant, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myb WT standard conditions Prim-25 hematopoietic stem cell ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 hematopoietic stem cell ISH
WT + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: forskolin Prim-25 hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 hematopoietic stem cell myb expression decreased amount, abnormal
Prim-25 hematopoietic stem cell differentiation disrupted, abnormal
Prim-25 prostaglandin-E synthase activity disrupted, abnormal
Prim-25 whole organism prostaglandin E2 decreased amount, abnormal
WT + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: forskolin Prim-25 hematopoietic stem cell myb expression amount, ameliorated
Prim-25 hematopoietic stem cell differentiation process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.