FIGURE

Fig. 4

ID

ZDB-FIG-160608-19

Publication

Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish

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Fig. 4

Defective proliferation and apoptosis caused by cyp2aa9MO were rescued by PGE2.

(a-d) cyp2aa9MO led to reduced number of mitotic active cells at 36 hpf in the AGM as shown by pH3 antibody staining, which could be rescued by PGE2. (e) Quantification of pH3-positive cells in the AGM (n = 6, mean ± SD). (f-i) cyp2aa9MO led to increases in the number of apoptotic cells at 36 hpf in the AGM as shown by TUNEL assay, which could be rescued by PGE2. (j) Quantification of the number of apoptotic cells in the AGM (n = 6, mean ± SD). *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test. All the views are exactly the AGM area shown in lateral, anterior left, dorsal top. Scale bar, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cyp2aa3,cyp2aa9
Condition:	chemical treatment by environment: prostaglandin E2
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Observed In:	ventral wall of dorsal aorta apoptotic process ventral wall of dorsal aorta cell population proliferation
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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