ZFIN ID: ZDB-FIG-160608-18
Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish. Scientific Reports   6:26608 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 3

PGE2 rescues defective HSC formation and reduced Wnt/β-catenin activity caused by cyp2aa9MO.

(a-d) Down-regulated Wnt/β-catenin activity in the VDA (brackets) caused by cyp2aa9MO was rescued by PGE2 as shown by WISH of GFP in the Tg(TOP:dGFP) Wnt-reporter line at 36 hpf. (e) Quantifications of total TOP-positive cells in the major trunk vessels (n = 6, mean ± SD). (f-i) Defective HSC formation in the cyp2aa9 morphants was rescued by PGE2 as shown by cmyb expression. (j-m) Decrease in the number of HSCs in the cyp2aa9 morphants was rescued by PGE2 as shown by the cmyb:GFP transgene. Scale bar, 50 µm. (n) Quantifications of the cmyb:GFP-positive cells in the AGM (n = 8, mean ± SD). *p < 0.05, ***p < 0.001, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf169Tg standard conditions Prim-25 hematopoietic stem cell IFL
zf169Tg + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 hematopoietic stem cell IFL
Prim-25 ventral wall of dorsal aorta IFL
zf169Tg + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: prostaglandin E2 Prim-25 hematopoietic stem cell IFL
GFP w25Tg standard conditions Prim-25 ventral wall of dorsal aorta ISH
w25Tg + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 ventral wall of dorsal aorta ISH
w25Tg + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: prostaglandin E2 Prim-25 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w25Tg + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 canonical Wnt signaling pathway disrupted, abnormal
Prim-25 ventral wall of dorsal aorta GFP expression decreased amount, abnormal
w25Tg + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: prostaglandin E2 Prim-25 canonical Wnt signaling pathway process quality, ameliorated
Prim-25 ventral wall of dorsal aorta GFP expression amount, ameliorated
zf169Tg + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 hematopoietic stem cell differentiation disrupted, abnormal
Prim-25 hematopoietic stem cell EGFP expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta EGFP expression decreased amount, abnormal
zf169Tg + MO1-cyp2aa3,cyp2aa9 chemical treatment by environment: prostaglandin E2 Prim-25 hematopoietic stem cell differentiation process quality, ameliorated
Prim-25 hematopoietic stem cell EGFP expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.