PGE2 rescues defective HSC formation and reduced Wnt/β-catenin activity caused by cyp2aa9MO.
(a-d) Down-regulated Wnt/β-catenin activity in the VDA (brackets) caused by cyp2aa9MO was rescued by PGE2 as shown by WISH of GFP in the Tg(TOP:dGFP) Wnt-reporter line at 36 hpf. (e) Quantifications of total TOP-positive cells in the major trunk vessels (n = 6, mean ± SD). (f-i) Defective HSC formation in the cyp2aa9 morphants was rescued by PGE2 as shown by cmyb expression. (j-m) Decrease in the number of HSCs in the cyp2aa9 morphants was rescued by PGE2 as shown by the cmyb:GFP transgene. Scale bar, 50 µm. (n) Quantifications of the cmyb:GFP-positive cells in the AGM (n = 8, mean ± SD). *p < 0.05, ***p < 0.001, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.