FIGURE

Fig. 3

ID

ZDB-FIG-160608-18

Publication

Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish

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Fig. 3

PGE2 rescues defective HSC formation and reduced Wnt/β-catenin activity caused by cyp2aa9MO.

(a-d) Down-regulated Wnt/β-catenin activity in the VDA (brackets) caused by cyp2aa9MO was rescued by PGE2 as shown by WISH of GFP in the Tg(TOP:dGFP) Wnt-reporter line at 36 hpf. (e) Quantifications of total TOP-positive cells in the major trunk vessels (n = 6, mean ± SD). (f-i) Defective HSC formation in the cyp2aa9 morphants was rescued by PGE2 as shown by cmyb expression. (j-m) Decrease in the number of HSCs in the cyp2aa9 morphants was rescued by PGE2 as shown by the cmyb:GFP transgene. Scale bar, 50 µm. (n) Quantifications of the cmyb:GFP-positive cells in the AGM (n = 8, mean ± SD). *p < 0.05, ***p < 0.001, Student’s t-test. All the views of embryos are lateral, anterior left, dorsal top.

Expression Data

Genes:	EGFP GFP
Fish:	w25Tg zf169Tg w25Tg + MO1-cyp2aa3,cyp2aa9 zf169Tg + MO1-cyp2aa3,cyp2aa9
Condition:	chemical treatment by environment: prostaglandin E2
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Anatomical Terms:	hematopoietic stem cell ventral wall of dorsal aorta
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w25Tg + MO1-cyp2aa3,cyp2aa9 zf169Tg + MO1-cyp2aa3,cyp2aa9
Condition:	chemical treatment by environment: prostaglandin E2
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Observed In:	canonical Wnt signaling pathway hematopoietic stem cell hematopoietic stem cell differentiation ventral wall of dorsal aorta
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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