Fig. 4

Somite/boundary markers are lost in mibⁿⁿ²⁰⁰² mutants. (A) WT embryos and (B) mibⁿⁿ²⁰⁰² mutants were stained with tbx24 at 12 hpf. (C) WT embryos and (D) mibⁿⁿ²⁰⁰² mutants were stained with papc at 12 hpf. (E) WT embryos and (F) mibⁿⁿ²⁰⁰² mutants were stained with myoD at 14 hpf. (G) WT embryos and (H) mibⁿⁿ²⁰⁰² mutants were stained with fgf8a at 14 hpf. (I) WT embryos and (J) mibⁿⁿ²⁰⁰² mutants were stained with dystrophin (dmd) at 14 hpf. (K) WT embryos and (L) mibⁿⁿ²⁰⁰² mutants were stained with tcf15 at 14 hpf, where DsRed filter was used to improve the signal to noise ratio of NBT/BCIP staining under transmitted light. All embryos were mounted in dorsal view with head to the top. tbx24 and papc were expressed in PSM, though the anterior segmental pattern was not observed in the region of nascent somites in mibⁿⁿ²⁰⁰² mutants. The expression of myoD, fgf8a, and dystrophin were not detected in the region where somites normally formed. tcf15 was weakly expressed in mibⁿⁿ²⁰⁰² mutants and no segmental pattern can be discerned.