FIGURE

Fig. 6

ID

ZDB-FIG-160517-24

Publication

Hsu et al., 2015 - A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.

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Fig. 6

mib gene is deleted in mibⁿⁿ²⁰⁰² mutants. (A) Diagram of the mutations of different mib alleles. The translation of mib in mibⁿⁿ²⁰⁰⁰ and mibⁿⁿ²⁰⁰¹ mutants stops at amino acids 5′ to ring finger domain 3 (RF3). (B) The linkage analysis result of mibⁿⁿ²⁰⁰² mutants. The upper part illustrates the locations of mib gene and the SSLP markers. The lower part demonstrates a close linkage of Z4662 and Z13620 to mib, with a recombination frequency of 0/97 and 2/214, respectively. (C) Real-time PCR results of mib expression in mibⁿⁿ²⁰⁰² homozygotes and WT embryos. The blue bars represent the expression level of mib in mibⁿⁿ²⁰⁰² homozygotes. The purple bars represent the expression level of mib in WT embryos. There is nearly no mib transcript detected in the mibⁿⁿ²⁰⁰² homozygotes. (D) WT embryos and (E) mibⁿⁿ²⁰⁰² mutants were stained with mib at 14 hpf, respectively. There is no mib mRNA detected in (E) mibⁿⁿ²⁰⁰² homozygous. (F) The results of mib genomic PCR were shown with fih (hif1an) as a positive control. AB represents AB WT samples; MU represents mibⁿⁿ²⁰⁰² homozygous samples; NE represents negative controls, where distilled water was used instead of genomic extracts as templates. The results showed that no mib genomic fragments are amplified.

Expression Data

Gene:	mib1
Fish:	WT Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Anatomical Term:	whole organism
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Observed In:	whole organism
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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