FIGURE

Fig. S3

ID
ZDB-FIG-160317-40
Publication
Liu et al., 2016 - Cannabinoid receptor signaling regulates liver development and metabolism
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Fig. S3

Examination of additional hepatic markers in cnr1-/- and cnr2-/- mutants

A) Representative in situ hybridization images and scatter plots quantifying (B-D) expression of additional hepatocyte markers in cnr1-/- and cnr2-/- mutants at 72hpf. Expression of group specific component (vitamin D binding protein, gc), secreted immunoglobulin domain 4 (sid4), and transferrin (tfa) were all reduced in cnr1-/- and cnr2-/- mutants. These results confirm smaller liver size and exclude a dependence on fabp10a expression when cannabinoid receptors are knocked out in these mutants. Data are represented as mean±s.e.m. with one-way ANOVA analysis. Results shown represent one independent experiment of triplicate experiments which yielded similar results, n >15 for each experiment, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Scale bar = 0.2 mm.

E) Representative in situ hybridization images and scatter plots quantifying (F-G) expression of hepatic progenitor markers hhex and prox1 at 72hpf. Hepatic progenitors remain unchanged in cnr1-/- and cnr2-/- mutants at 72hpf. These data indicate that disruption of signaling through cannabinoid receptors impacts hepatic differentiation. Data are represented as mean±s.e.m. with one-way ANOVA analysis. Results shown represent one independent experiment of triplicate experiments which yielded similar results, n >15 for each experiment, p=0.42 and p=0.68 for hhex and prox1, respectively. Scale bar = 0.2 mm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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