FIGURE

Fig. S1

ID

ZDB-FIG-160317-38

Publication

Liu et al., 2016 - Cannabinoid receptor signaling regulates liver development and metabolism

Other Figures

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Fig. S1

Cannabinoid receptor expression in the developing zebrafish

A) FACS quantification of GFP-positive hepatocytes in fabp10a:GFP embryos treated with cannabinoid agonists from 24-72hpf. % of GFP positive cells were normalized to controls. Mean±s.e.m., n=5 groups of 10 pooled embryos; p=0.08, one-way ANOVA analysis.

B) Dorsal and lateral in situ hybridization images at 24, 48, and 72hpf showing that cnr1 and cnr2 are expressed in the developing central nervous system, endoderm (arrow), and liver regions (arrow). Scale bar = 0.2 mm.

C) qRT-PCR detects cnr1 expression in pooled FACS-sorted GFP-positive cells (~200,000 cells) from wild type fabp10a:GFP normalized to expression in the GFP-negative cell fraction, which includes the central nervous system. cnr2 is detectable in the non-hepatocyte (GFP-negative) fraction, but not in the hepatocytes.

Expression Data

Genes:	cnr1 cnr2
Fish:	WT as3Tg
Anatomical Terms:	central nervous system hepatocyte liver liver anatomical region
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	as3Tg
Condition:	chemical treatment: cannabinoid receptor agonist
Observed In:	hepatocyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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