ZFIN Image: Bühler et al., 2016, Fig. S1

Image

Figure Caption

Fig. S1

(A) RT-PCR for atrogin-1 and β-actin on wild-type on zebrafish cDNA from 12-Somite stage (S) until 72 hpf; (B) Western blot analysis using an anti-Myc antibody after atrogin-1 mRNA and MO2-atrogin-1 + atrogin-1 mRNA injection; (C) Immunoblot analysis using an anti-Atrogin-1 antibody after MO1-control and MO2-atrogin-1 injection; (D,E) Lateral views of control injected (D) and MO1-atrogin-1 injected embryos (E) showing birefringence at 72 hpf; (F-I) Electron micrographs of skeletal muscle cells (skm) of Atrogin-1 morphants (G,I) and embryos injected with control Morpholino (F,H) at 72 hpf. (J,J′) Immunofluorescent staining against Atrogin-1 on paraffin sections, in lateral view of wild-type embryo at 72 hpf, nuclei are counterstained with DAPI.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.