FIGURE

Fig. S9

ID

ZDB-FIG-151211-5

Publication

Jia et al., 2015 - Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction

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Fig. S9

Analysis of total protein and polysome assembly in WT and kri1l^cas002 embryos. (A-C) Total protein was extracted from lysates of deyolked whole embryos (n>20) and then measured by BCA (bicinchoninic acid) protein assay. The protein synthesis was impaired at 3 dpf (p=0.0003), 4 dpf (p<0.0001) and 5 dpf (p<0.0001). Error bars represent standard error of the mean (SEM). ***, P≤0.001 (Student t test). (D) Representative polysome fractionation analysis performed on WT and kri1l^cas002 embryos at 3 dpf demonstrates reduced levels of 40 S ribosomal subunits, 80 S monosomes and polysomes; and excessive 60 S subunits in kri1l^cas002 larvae compared to that in WT.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kri1^{cas002/cas002}
Observed In:	whole organism cytosolic large ribosomal subunit whole organism cytosolic ribosome whole organism cytosolic small ribosomal subunit whole organism ribosome
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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