FIGURE

Fig. S18

ID

ZDB-FIG-151009-43

Publication

Jia et al., 2015 - Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction

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Fig. S18

Z36 antagonizes Bcl2-mediated rescue effect on hematopoiesis defects in kri1l^cas002 mutants. kri1l^cas002 mutant embryos injected with bcl2 mRNA were treated with Z36 (10 µm) from 36 hpf for 36 hours. Hematopoietic phenotype was classified and quantified (D) based on cmyb WISH analysis and representative images are shown in (A-C). (E-J) Z36 recovers excessive autophagy in kri1l^cas002 mutants injected with bcl2 mRNA. mCherry–Lc3 puncta were counted over 20 EGFP⁺ cells (in the Tg(cmyb:egfp) background) in 8-11 individual embryos. Quantitative results were summarized in (K). Error bars in K represent standard error of the mean (SEM). **, P≤0.01; ***, P≤0.001 (Student t test). Scale bars, 5 µm. (L) Quantitation of Lc3-mCherry+ autophagosome in Figure 6M-R. The number of autophagosome was decreased in kri1l^cas002 embryos after GSK2656157 treatment, p=0.0091.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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