FIGURE

Fig. S16

ID

ZDB-FIG-151009-41

Publication

Jia et al., 2015 - Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction

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Fig. S16

The comparison of autophagic level between kri1l^cas002 mutants and wild-type siblings during hematopoietic development. Representative confocal images of mCherry–Lc3 puncta (autophagosomes) in HSPCs of live zebrafish embryos in different time points and regions, including the AGM at 40 hpf (A-F) (Scale bars: 5 µm.); the CHT at 40 hpf (G-L) (Scale bars: 5 µm.), and at 2 dpf (M-R) (Scale bars: 5 µm.). (S) Statistic analysis of the autophagy index (the average number of autophagosomes per HSPC) for kri1l^cas002 mutants and wild-type siblings during hematopoietic development (40 hpf-2 dpf) mCherry–Lc3 puncta were counted over 20 EGFP+ cells (in the Tg(cmyb:egfp) background) in 5-8 individual embryos under each condition. 40 hpf AGM, p=0.4272; 40 hpf CHT, p<0.0001; 2 dpf CHT, p<0.0001. Error bars represent standard error of the mean (SEM). **, P0.01; ***, P≤0.001 (Student t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kri1^{cas002/cas002}; zf169Tg
Observed In:	blood island autophagy
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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