FIGURE

Fig. 5, S1

ID

ZDB-FIG-151124-12

Publication

Agarwala et al., 2015 - Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish

Other Figures

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Fig. 5, S1

yap1 and taz are ubiquitously expressed at 30 hpf.

Zyap1^/ mutant embryos have fewer cells compared to sibling controls. (A–B′′) 30 hpf cldnb:gfp embryos stained with a yap1 (A–A′′) or a taz (B–B′′) ISH probe and an anti-GFP antibody (A′′, B′′). (C–E, H) Boxplot showing pLLP cell counts (C), number of deposited neuromasts (D), cell counts (E), and number of hair cells (H) per neuromast in the indicated experimental conditions. Cell counts were performed in the neuromast closest to the end of yolk extension (n3 or n4) at 72 hpf in (E–H). (F–G′′) MIP of Z-stacks of a deposited neuromast (n3 or n4) stained with an anti-HCS1 antibody (hair cells, red) and DAPI (cell nuclei, blue). (I–L′) ISH with a prox1 probe and an anti-GFP antibody staining (J′, L′) on embryos with the indicated genetic background. Red arrows point to the pLLP (A, B, I and K) (Figure 5—source data 3, 4).

Expression Data

Genes:	EGFP prox1a wwtr1 yap1
Antibody:	Ab1-otof
Fish:	zf106Tg yap1^fu47/fu47; zf106Tg
Anatomical Terms:	posterior lateral line neuromast neuromast hair cell posterior lateral line primordium
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	yap1^fu47/fu47; zf106Tg yap1^fu47/fu47; zf106Tg
Observed In:	neuromast hair cell posterior lateral line posterior lateral line neuromast posterior lateral line neuromast cell posterior lateral line primordium posterior lateral line primordium cell
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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