FIGURE

Fig. 2

ID

ZDB-FIG-151124-4

Publication

Agarwala et al., 2015 - Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish

Other Figures

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Fig. 2

Amotl2a is not essential for proneuromast assembly but for proper migration.

(A–F) MIP of Z-stacks of pLLP stained with ZO-1 (blue) and GFP (green) antibodies and phalloidin (red) in control (A, C, E) and amotl2a morphant (B, D, F) cldnb:gfp embryos at 30 hpf. (G, H) MIP of overview images of control (G) and amotl2a morphant (H) embryos after completion of migration. (I, J) Snapshots of time-lapse movies at the indicated timepoints showing a delay in migration in amotl2a morphants (J) as compared to controls (I). (K, L) Corresponding kymographs used to measure the migration speed. (M) Boxplot comparing the migration speeds (Figure 2—source data 1, Figure 2—figure supplements 1, 2).

Expression Data

Gene:	EGFP
Antibody:	Ab1-tjp1
Fish:	zf106Tg zf106Tg + MO1-amotl2a
Knockdown Reagent:	MO1-amotl2a
Anatomical Terms:	posterior lateral line primordium posterior lateral line primordium bicellular tight junction
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg + MO1-amotl2a
Knockdown Reagent:	MO1-amotl2a
Observed In:	posterior lateral line primordium posterior lateral line neuromast primordium migration
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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