ZFIN ID: ZDB-FIG-151102-32
Ghaye et al., 2015 - Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration. BMC Biology   13:70 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: 10-13 somites to Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: 10-13 somites to Day 5

Fig. 5

ascl1b and nkx6.1 are regulated in an opposite way by the Notch signaling pathway. af WISH on wild-type (WT) embryos (a, c, e) or mind bomb (mib-/-) mutants (b, d, f) with ascl1b (a, b) or nkx6.1 (c–f) probes. Brackets delimit the pancreatic domain. g, h Immunodetection of GFP and endogenous Nkx6.1 in 5-dpf Tg(ascl1b:eGFP-creER T2 ) embryos treated from 3 to 5 dpf with DMSO (g) or with the Notch signaling inhibitor, LY411575 (h). in WISH with ascl1b (i, j) or nkx6.1 (k, l) probes or immunodetection of endogenous Nkx6.1 (m, n) of double-transgenic Tg(hsp70:Gal4) x Tg(UAS:NICD) embryos (j, l, n) or of control simple-transgenic embryos (i, k, m), both heat-shocked at 11 hpf for 20 min. Lateral views (af, k, l) or ventral views (gj, m, n) of embryos of the indicated stages with the anterior to the left. Scale bars = 40 µm

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1b AB standard conditions 10-13 somites nervous system ISH
10-13 somites pancreas primordium ISH
kca3Tg; kca4Tg heat shock 10-13 somites Not Detected pancreas primordium ISH
mib1ta52b/ta52b standard conditions 10-13 somites nervous system ISH
10-13 somites pancreas primordium ISH
EGFP ulg006Tg(AB) control Day 5 endocrine pancreas IHC
ulg006Tg(AB) chemical treatment: LY-411575 Day 5 endocrine pancreas IHC
nkx6.1 AB standard conditions 10-13 somites nervous system ISH
10-13 somites pancreas primordium ISH
14-19 somites nervous system ISH
14-19 somites pancreas primordium ISH
kca3Tg; kca4Tg heat shock 10-13 somites pancreas primordium ISH
mib1ta52b/ta52b standard conditions 10-13 somites nervous system ISH
10-13 somites pancreas primordium ISH
14-19 somites nervous system ISH
14-19 somites Not Detected pancreas primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-nkx6 IHC kca3Tg; kca4Tg heat shock Prim-15 pancreatic bud
IHC ulg006Tg(AB) control Day 5 pancreatic duct
IHC ulg006Tg(AB) chemical treatment: LY-411575 Day 5 Not Detected pancreatic duct
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg heat shock 10-13 somites pancreas primordium ascl1b expression absent, abnormal
10-13 somites pancreas primordium nkx6.1 expression increased amount, abnormal
Prim-15 pancreatic bud ab2-nkx6 labeling increased distribution, abnormal
mib1ta52b/ta52b standard conditions 10-13 somites nervous system ascl1b expression increased amount, abnormal
10-13 somites nervous system nkx6.1 expression decreased amount, abnormal
10-13 somites pancreas primordium ascl1b expression increased amount, abnormal
10-13 somites pancreas primordium nkx6.1 expression decreased amount, abnormal
14-19 somites nervous system nkx6.1 expression decreased amount, abnormal
14-19 somites pancreas primordium nkx6.1 expression absent, abnormal
ulg006Tg(AB) chemical treatment: LY-411575 Day 5 endocrine pancreas EGFP expression increased distribution, abnormal
Day 5 pancreatic duct ab2-nkx6 labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.