Co-expression of klf8 and pax6a is detected in the retinae, thalamus, and hindbrain, and co-expression of klf8 and otx2 is observed in the midbrain. (A-F) Lateral views of embryos are shown. Expression of klf8 (red; A, D) and pax6a (green; B, E) in embryos at 24 hpf; merged images are shown in (C) and (F). (G-L) Dorsal views of embryos are shown. Expression of klf8 (red; G) and otx2 (green; H) in embryos at 24 hpf; merged images are shown in (I). Expression of klf8 (red, J) and nkx6.1 (green, K) in embryos at 24 hpf; merged images are shown in (L). H, hindbrain; mb, midbrain; r, retina; th, thalamus; vh, ventral hindbrain; vmb, ventral midbrain.
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