Co-expression of klf8 and met in the tectum, R1, and hindbrain, and co-expression of klf8 and ptf1a in the VZ. (A-I) Expression of klf8 (red; A, D, G) and met (green; B, E, H) in embryos at 24 hpf; merged images are shown in (C, F) and (I). Lateral views of embryos are shown. (J-L) Dorsal views of Tg(ptfla:EGFP)jh1 transgenic fish embryos following double fluorescence in situ hybridization. Expression of klf8 (red; J) and ptf1a (as visualized in green using a GFP probe; K) in embryos at 48 hpf; a merged image is shown in (L). Single confocal images are shown. H, hindbrain; r1, rhombomere 1; te, tectum; VZ, ventricular zone.
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