Fig. 4

Knockdown of klf8 eliminates ptf1a expression in the VZ and affects the development of Purkinje cells. (A–D) Expression of ptf1a in the VZ at 48 hpf is decreased in embryos injected with klf8-MO1^atg (C, arrowhead) or co-injected with klf8-MO^AC and klf8-MO^DO (D, arrowhead), but expression is observed in the VZ of WT embryos (A) or embryos injected with klf8-4mmMO1 (B). (E) Percentage of embryos of the indicated backgrounds showing decreased ptf1a expression in the VZ at 48 hpf. (F-I) Expression of ptf1a in the VZ at 72 hpf is decreased in embryos injected with klf8-MO1^atg (H, arrowhead) or co-injected with klf8-MO^AC and klf8-MO^DO (I, arrowhead), but expression is observed in the VZ of WT embryos (F) or embryos injected with klf8-4mmMO1 (G). (J) Percentage of embryos of the indicated backgrounds showing decreased ptf1a expression in the VZ at 72 hpf. (K-N) Expression of roraa in the VZ at 72 hpf is not detected in embryos injected with klf8-MO1^atg (M, arrowhead) or co-injected with klf8-MO^AC and klf8-MO^DO (N, arrowhead), but expression is observed in the VZ of WT embryos (K) or embryos injected with klf8-4mmMO1 (L). (O) Percentage of embryos of the indicated backgrounds lacking roraa expression in the VZ at 72 hpf. (P–S) Expression of Pvalb7 in the VZ at 72 hpf is not detected in embryos injected with klf8-MO1^atg (R) or co-injected with klf8-MO^AC and klf8-MO^DO (S), but expression is observed in the VZ of WT embryos (P) or embryos injected with klf8-4mmMO1 (Q). (T) Percentage of embryos of the indicated backgrounds lacking Pvalb7 expression at 72 hpf. Dorsal views of embryos are shown. Significance was determined using Student′s t-test. ***p < 0.001. Error bars indicate standard errors. Pc, Purkinje cells; VZ, ventricular zone.