FIGURE

Fig. 6

ID

ZDB-FIG-151008-20

Publication

Hu et al., 2015 - Loss of DDB1 Leads to Transcriptional p53 Pathway Activation in Proliferating Cells, Cell Cycle Deregulation, and Apoptosis in Zebrafish Embryos

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Fig. 6

Knock down of p53 rescued the apoptosis phenotype of ddb1^m863 mutants.

(A-F) Enhanced apoptosis in ddb1^m863 (class I phenotype; D-F) compared to wild type siblings (A-C) without p53_MO injection. (G-R) Partial (class II and class III; J-O) and complete (type IV; P-R) rescue of ddb1^m863 apoptosis phenotype compared to wild siblings (G-I) after injection of 12 ng p53_MO per embryo. (S) Percentage distribution of mutant and rescued mutant phenotypes. Genotypes were determined by PCR. Class I (orange), mutants showed the severest apoptosis phenotype; class IV (light blue) mutants have the weakest phenotype with only a few of apoptotic cells, similar to their wild type siblings; class II (yellow) and III (green) are intermediate phenotypes. Abbreviations used: tpz, tectal proliferation zone; Cce, cerebellum; ret, retina; Anterior towards the left. Scale bar: 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddb1^m863/m863 WT + MO4-tp53 ddb1^m863/m863 + MO4-tp53
Knockdown Reagent:	MO4-tp53
Observed In:	cerebellum apoptotic process ciliary marginal zone apoptotic process optic tectum apoptotic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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