ZFIN ID: ZDB-FIG-150923-15
Yan et al., 2015 - Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. Journal of hepatology   63(2):420-8 Full text @ J. Hepatol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 6

Fig. S1 Effect of morpholino knockdown of Gcsfr and Irf8. (A) 6-dpf lyz+ larvae injected with morpholinos MO-SC (top), MO-gcsfr (middle) or MO_irf8 (bottom). (B) 6-dpf mpeg+ larvae injected with morpholinos MO-SC (top), MO-gcsfr (middle) or MO_irf8 (bottom). (C) Quantification of number of neutrophils per larva. (D) Quantification of number of microphages per larva. (n=5, *p<0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 nz50Tg control Day 6 neutrophil IFL
nz50Tg + MO1-irf8 standard conditions Day 6 neutrophil IFL
nz50Tg + MO3-csf3r standard conditions Day 6 neutrophil IFL
EGFP gl22Tg control Day 6 macrophage IFL
gl22Tg + MO1-irf8 standard conditions Day 6 macrophage IFL
gl22Tg + MO3-csf3r standard conditions Day 6 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl22Tg + MO1-irf8 standard conditions Day 6 macrophage decreased amount, abnormal
gl22Tg + MO3-csf3r standard conditions Day 6 macrophage increased amount, abnormal
nz50Tg + MO1-irf8 standard conditions Day 6 neutrophil increased amount, abnormal
nz50Tg + MO3-csf3r standard conditions Day 6 neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of hepatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Journal of hepatology, 63(2), Yan, C., Huo, X., Wang, S., Feng, Y., Gong, Z., Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish, 420-8, Copyright (2015) with permission from Elsevier. Full text @ J. Hepatol.