ZFIN ID: ZDB-FIG-150923-10
Yan et al., 2015 - Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. Journal of hepatology   63(2):420-8 Full text @ J. Hepatol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 6

Fig. 3

Effect of morpholino suppression of neutrophil differentiation and morpholino-mediated expansion of neutrophil population on liver size. (A–C) Representative images of 6 dpf kras-/lyz+ and kras+/lyz+ larvae injected with different morpholinos: Mo-SC (A), Mo-gcsfr (B) or Mo-irf8 (C). (D–F) Neutrophil counts (D) and density (E) in the liver area and liver size (F) after morpholino injection. n >15 in each group. Statistical significant: *p <0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gz32Tg; nz50Tg chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
gz32Tg; nz50Tg + MO1-irf8 chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
gz32Tg; nz50Tg + MO3-csf3r chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
nz50Tg chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
nz50Tg + MO1-irf8 chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
nz50Tg + MO3-csf3r chemical treatment: doxycycline monohydrate Day 6 neutrophil IFL
EGFP gz32Tg; nz50Tg chemical treatment: doxycycline monohydrate Day 6 liver IFL
gz32Tg; nz50Tg + MO1-irf8 chemical treatment: doxycycline monohydrate Day 6 liver IFL
gz32Tg; nz50Tg + MO3-csf3r chemical treatment: doxycycline monohydrate Day 6 liver IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz32Tg; nz50Tg chemical treatment: doxycycline monohydrate Day 6 liver increased size, abnormal
Day 6 liver neutrophil increased amount, abnormal
gz32Tg; nz50Tg + MO1-irf8 chemical treatment: doxycycline monohydrate Day 6 liver increased size, abnormal
Day 6 liver neutrophil increased amount, abnormal
nz50Tg + MO3-csf3r chemical treatment: doxycycline monohydrate Day 6 liver neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of hepatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Journal of hepatology, 63(2), Yan, C., Huo, X., Wang, S., Feng, Y., Gong, Z., Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish, 420-8, Copyright (2015) with permission from Elsevier. Full text @ J. Hepatol.