FIGURE

Fig. 5

ID

ZDB-FIG-150430-13

Publication

Luz et al., 2014 - Dynamic Association with Donor Cell Filopodia and Lipid-Modification Are Essential Features of Wnt8a during Patterning of the Zebrafish Neuroectoderm

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Wnt8a mutations affect cell-non-autonomous neural plate patterning activity.

(A) Scheme showing transplantation of cells expressing Wnt8a-GFP or Wnt8a-GFP mutated proteins. (B) Wnt8a-GFP expressing cells (brown) transplanted into a WT embryo at shield stage are able to repress otx2 (blue) in the surrounding neural plate at 80% epiboly stage (2 hours post transplantation. (A′–F′) Expression of otx2 before (B–F′) and after (A′–F′) detection of transplanted cells. Both Wnt8aCA-GFP and Wnt8aSA-GFP are able to repress otx2 (B,C) but only cell-autonomously. Wnt8aCASA-GFP, Wnt8aCYSA-GFP and Wnt8aCY-GFP expressing cells fail to repress otx2 both non- and cell-autonomously. Orientation is anterior to the to the top (D–F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One