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Fig. 5
Wnt8a mutations affect cell-non-autonomous neural plate patterning activity.
(A) Scheme showing transplantation of cells expressing Wnt8a-GFP or Wnt8a-GFP mutated proteins. (B) Wnt8a-GFP expressing cells (brown) transplanted into a WT embryo at shield stage are able to repress otx2 (blue) in the surrounding neural plate at 80% epiboly stage (2 hours post transplantation. (A′–F′) Expression of otx2 before (B–F′) and after (A′–F′) detection of transplanted cells. Both Wnt8aCA-GFP and Wnt8aSA-GFP are able to repress otx2 (B,C) but only cell-autonomously. Wnt8aCASA-GFP, Wnt8aCYSA-GFP and Wnt8aCY-GFP expressing cells fail to repress otx2 both non- and cell-autonomously. Orientation is anterior to the to the top (D–F).