FIGURE

Fig. 3

ID

ZDB-FIG-150428-13

Publication

Hoyle et al., 2013 - Transcript processing and export kinetics are rate-limiting steps in expressing vertebrate segmentation clock genes

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Fig. 3

Estimation of transcript export and splicing delays of Hes7 in the mouse PSM. (A, i) Maximum z-projection of FISH against Mouse Hes7. Segmentation of higher magnification FISH images was conducted as in Fig. 2B. An example source image (ii) and detailed view of the source image indicated by the hatched area (ii2) are shown, along with the segmented images representing unspliced nuclear pre-mRNA (iii), spliced nuclear mRNA (iv), and spliced cytoplasmic mRNA (v). Average z-projections are shown. (Scale bar: 100 µm.) (B) Intensity plots of images in A, iii–v measured from the posterior to anterior of the expression domain, averaged across the width of the PSM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA