Fig. 5
- ID
- ZDB-FIG-150413-12
- Publication
- Ma et al., 2015 - Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death
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The anterior and lateral cristae are developed from a single anterior prosensory domain. (A-B′′′) Time-lapse imaging of the anterior pro-sensory domain development in jag1b+/- and jag1b-/- embryos. In jag1b-/- embryos, ACP and cells in the middle of the anterior prosensory domain undergo morphological change (B′′,B′′′, arrows). ACP, anterior crista primordia; LCP, lateral crista primordia; AC, anterior crista; LC, lateral crista. Arrows and arrowheads indicate cells undergoing morphological change. (C-F′) E-cadherin immunofluorescence staining in jag1b+/- and jag1b-/- embryos. At 40hpf, only cells in the middle of the anterior prosensory domain express E-cadherin in jag1b+/- embryos (D,D′, arrowheads). In jag1b-/- embryos, ACP and cells in the middle of the anterior prosensory domain express E-cadherin (F,F′, arrows). (G,H) Treatment with FGF inhibitor SU5402 at 32-34hpf blocks the separation of anterior and lateral cristae in jag1b+/- embryos. (I-J′) pErk3 immunofluorescent staining in jag1b+/- and jag1b-/- embryos. Dorsolateral views. Scale bars: 20µm. Anterior towards the left and dorsal upwards. Arrows and arrowheads indicate staining of E-Cadherin or p-Erk3. |
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| Stage Range: | Prim-15 to Long-pec |