ZFIN Image: Ma et al., 2015, Fig. 5

Image

Figure Caption

Fig. 5

The anterior and lateral cristae are developed from a single anterior prosensory domain. (A-B′′′) Time-lapse imaging of the anterior pro-sensory domain development in jag1b^+/- and jag1b^-/- embryos. In jag1b^-/- embryos, ACP and cells in the middle of the anterior prosensory domain undergo morphological change (B′′,B′′′, arrows). ACP, anterior crista primordia; LCP, lateral crista primordia; AC, anterior crista; LC, lateral crista. Arrows and arrowheads indicate cells undergoing morphological change. (C-F′) E-cadherin immunofluorescence staining in jag1b^+/- and jag1b^-/- embryos. At 40hpf, only cells in the middle of the anterior prosensory domain express E-cadherin in jag1b^+/- embryos (D,D′, arrowheads). In jag1b^-/- embryos, ACP and cells in the middle of the anterior prosensory domain express E-cadherin (F,F′, arrows). (G,H) Treatment with FGF inhibitor SU5402 at 32-34hpf blocks the separation of anterior and lateral cristae in jag1b^+/- embryos. (I-J′) pErk3 immunofluorescent staining in jag1b^+/- and jag1b^-/- embryos. Dorsolateral views. Scale bars: 20µm. Anterior towards the left and dorsal upwards. Arrows and arrowheads indicate staining of E-Cadherin or p-Erk3.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development