ZFIN ID: ZDB-FIG-150331-50
Moreno et al., 2014 - Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability. Neural Development   9:19 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 26+ somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 26+ somites to Prim-5

Fig. 1

Caudal primary motor neuron-like neurons have abnormal axonal trajectories that resemble those of interneurons. In all figures, unless indicated otherwise, images present lateral views of embryos (rostral left, dorsal up). (A) In control (Ctl) Tg(mnx1:gfp)ml2 22 to 26 hours post-fertilization (hpf) embryos, primary motor neurons (PMNs) express green fluorescent protein (GFP). The caudal PMN (CaP; large white arrowhead) projects its peripheral axon (small white arrowhead) ventrally. White lines (A, B and C) indicate somite boundaries. (B) In Tg(mnx1:gfp)ml2 E3 morphants, GFP+ cells persist in the ventral spinal cord. GFP+ CaP-like cells (yellow arrowhead) have somas in CaP positions but project axons centrally rather than peripherally. (C) In another Tg(mnx1:gfp)ml2 E3 morphant, a GFP+ CaP-like cell (yellow arrowhead), filled with Alexa 594, has an axon that extends caudally to exit in the neighboring hemisegment (thin yellow arrowhead). (D-L) (D) In uninjected Tg(nrp1a:gfp)js12 embryos, CaP (white arrowhead) has its soma immediately dorsal to the motor axon exit point (white asterisk). (E) E3 morphants have few GFP+ ventral neurons. A CaP-like cell (yellow arrowhead) lacks a peripheral axon. (F, F′) GFP+ PMNs of control Tg(nrp1a:gfp)js12 embryos (F′, white asterisks) express nrp1a. (G, G′) Following Islet1 knock-down, few nrp1a/GFP+ (G′, yellow asterisks) neurons are present. (H-L) In E3 morphants, many GFP+ neurons have axons that bypass normal exit points and extend centrally either caudally (H and I), rostrally (J) or in both directions (K). Occasionally, a GFP+ CaP-like cell extends a peripheral as well as a central axon (L). (M) The top cartoon depicts control CaP axon morphology, and the six lower cartoons exemplify the range of CaP-like axonal phenotypes revealed by either dye filling or confocal analysis of Tg(nrp1a:gfp)js12 E3 morphants. Scale bars = 50 ┬Ám in A (for A to C), D (for D and E), F (for F to G′) and H (for H to L). IC, ipsilateral commissural; KA, Kolmer-Agduhr; VeLD, ventral lateral.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP js12Tg control 26+ somites to Prim-5 CaP motoneuron IHC
26+ somites to Prim-5 CaP motoneuron axon IHC
26+ somites to Prim-5 primary motor neuron IHC
26+ somites to Prim-5 primary motor neuron axon IHC
js12Tg + MO3-isl1a standard conditions 26+ somites to Prim-5 CaP motoneuron IHC
26+ somites to Prim-5 CaP motoneuron axon IHC
26+ somites to Prim-5 primary motor neuron IHC
26+ somites to Prim-5 primary motor neuron axon IHC
ml2Tg control 26+ somites to Prim-5 CaP motoneuron IFL
26+ somites to Prim-5 CaP motoneuron axon IFL
26+ somites to Prim-5 primary motor neuron IFL
26+ somites to Prim-5 primary motor neuron axon IFL
ml2Tg + MO3-isl1a standard conditions 26+ somites to Prim-5 CaP motoneuron IFL
26+ somites to Prim-5 CaP motoneuron axon IFL
26+ somites to Prim-5 primary motor neuron IFL
26+ somites to Prim-5 primary motor neuron axon IFL
nrp1a js12Tg control 26+ somites to Prim-5 CaP motoneuron ISH
26+ somites to Prim-5 CaP motoneuron axon ISH
26+ somites to Prim-5 primary motor neuron ISH
26+ somites to Prim-5 primary motor neuron axon ISH
js12Tg + MO3-isl1a standard conditions 26+ somites to Prim-5 CaP motoneuron ISH
26+ somites to Prim-5 CaP motoneuron axon ISH
26+ somites to Prim-5 primary motor neuron ISH
26+ somites to Prim-5 primary motor neuron axon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
js12Tg + MO3-isl1a standard conditions 26+ somites - Prim-5 CaP motoneuron neuron projection displaced, abnormal
26+ somites - Prim-5 CaP motoneuron neuron projection morphology, abnormal
ml2Tg + MO3-isl1a standard conditions 26+ somites - Prim-5 CaP motoneuron neuron projection displaced, abnormal
26+ somites - Prim-5 CaP motoneuron neuron projection morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.