ZFIN ID: ZDB-FIG-150331-52
Moreno et al., 2014 - Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability. Neural Development   9:19 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3

Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B), Rohon-Beard cells (RBs) express runx3. Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F′)Tg(-3.4neurog1:gfp)sb4 control (D-E′) and E3 morphant (F, F′) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E′) In lateral views of the dorsal spinal cord, RNA in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP)+ neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure  2). (F-F′) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E′), RB-like neurons do not express detectable levels of olig4(F). (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP+ neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP+ DRGs. Furthermore, for the few GFP+ DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 ┬Ám in A (for A-C), D (for D-F′) and G (for G-I). CtlMO, 5-base mismatched; islet1(Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP sb4Tg control Prim-5 DoLA IHC
Prim-5 Rohon-Beard neuron IHC
Prim-5 spinal cord interneuron IHC
Long-pec dorsal root ganglion IFL
sb4Tg standard conditions Prim-5 DoLA IHC
Prim-5 Rohon-Beard neuron IHC
Prim-5 spinal cord interneuron IHC
Long-pec dorsal root ganglion IFL
sb4Tg + MO3-isl1a standard conditions Prim-5 DoLA IHC
Prim-5 Rohon-Beard neuron IHC
Prim-5 spinal cord interneuron IHC
Long-pec dorsal root ganglion IFL
olig4 sb4Tg control Prim-5 Not Detected Rohon-Beard neuron ISH
Prim-5 spinal cord interneuron ISH
sb4Tg standard conditions Prim-5 Not Detected Rohon-Beard neuron ISH
Prim-5 spinal cord interneuron ISH
sb4Tg + MO3-isl1a standard conditions Prim-5 Not Detected Rohon-Beard neuron ISH
Prim-5 spinal cord interneuron ISH
runx3 WT control Prim-5 Rohon-Beard neuron ISH
WT standard conditions Prim-5 Rohon-Beard neuron ISH
WT + MO3-isl1a standard conditions Prim-5 Rohon-Beard neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-isl1a standard conditions Prim-5 Rohon-Beard neuron decreased amount, abnormal
sb4Tg + MO3-isl1a standard conditions Long-pec dorsal root ganglion decreased amount, abnormal
Long-pec dorsal root ganglion neuron projection morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.