FIGURE

Fig. 1

ID

ZDB-FIG-150224-6

Publication

Berger et al., 2014 - Loss of tropomodulin4 in the zebrafish mutant tr�ge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy

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Fig. 1

The mutant tr�ge (trg) shows a reduction in birefringence, indicating muscle damage. (A) Under brightfield microscopy, trg mutants appear similar to their wild-type siblings. (B) Under polarised light, the muscle of siblings appears brighter than that of the (B′) trg mutants due to a reduction in birefringence. (C) Quantification of the birefringence followed by normalization to that of 3-dpf-old siblings reveals that the birefringence of the siblings increases from 3 dpf to 6 dpf, roughly following a sigmoidal curve. By contrast, 3-dpf- to 6-dpf-old trg larvae show a highly significant reduction in birefringence when compared with that of 3-dpf siblings (P<0.01, n=3). (D) Immunohistochemistry with antibodies against dystrophin shows that dystrophin expression at the vertical myosepta (arrowheads) is unaffected in trg mutants. Data are means � s.e.m., **P<0.01. Scale bar: 200 μm.

Expression Data

Gene:	dmd
Antibody:	Ab1-dmd
Fish:	WT tmod4^pc20/pc20
Anatomical Term:	vertical myoseptum
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmod4^pc20/pc20
Observed In:	myotome skeletal muscle myofibril skeletal myofibril assembly vertical myoseptum
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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