ZFIN ID: ZDB-FIG-150224-9
Berger et al., 2014 - Loss of tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy. Disease models & mechanisms   7(12):1407-15 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 3 Muscle phenotype of tmod4trg. (A) In comparison to their siblings, (A′) myofibres in H&E-stained cross sections of tmod4trg larvae show less defined cell shapes and inhomogeneous eosin staining at 3 dpf. (B,B′) Magnified views of the respective boxes indicated in A and A′. (C,C′) Accordingly, myofibres on sagittal sections of tmod4trg mutants appear unstructured, and blue haematoxylin-stained nuclei (arrow) are rounder in shape compared with those of siblings. (D) In a sagittal view of 3-dpf-old siblings, the myofibrils show typical striation, when marked by using the transgenic line Tg(acta1:lifeact-GFP). In addition, the transgenic background Tg(acta1:mCherryCAAX) labels the sarcolemma in red, showing that myofibrils occupy most of the myofibres. (D′) In matching tmod4trg mutants, the myofibril striation is rarely seen (arrowhead in E′), instead myofibres are filled with misorientated thin filaments (arrow in E′). (E,E′) Magnifications of respective boxes in D and D′. (F,F′) GFP highlights the cephalic musculature in the transgenic background of Tg(-503unc:GFP), here shown as ventral views of z-stack projections. (F) In contrast to their siblings, (F′) the two hyohyoideus (hh) muscles in tmod4trg mutants leave a gap between each other (asterisk). (G) Focus stacks of larvae that were stained with Alcian blue and Alizarin red depict cartilage malformations in tmod4trg at 6 dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pc11Tg standard conditions Protruding-mouth cephalic musculature IFL
pc21Tg; pc22Tg standard conditions Protruding-mouth skeletal muscle cell striated muscle thin filament IFL
tmod4pc20/pc20; pc11Tg standard conditions Protruding-mouth cephalic musculature IFL
tmod4pc20/pc20; pc21Tg; pc22Tg standard conditions Protruding-mouth skeletal muscle cell striated muscle thin filament IFL
mCherry pc21Tg; pc22Tg standard conditions Protruding-mouth skeletal muscle cell sarcolemma IFL
tmod4pc20/pc20; pc21Tg; pc22Tg standard conditions Protruding-mouth skeletal muscle cell sarcolemma IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tmod4pc20/pc20 standard conditions Day 6 splanchnocranium malformed, abnormal
tmod4pc20/pc20; pc11Tg standard conditions Protruding-mouth hyohyoideus misaligned away from hyohyoideus, abnormal
tmod4pc20/pc20; pc21Tg; pc22Tg standard conditions Protruding-mouth myotome striated muscle thin filament disoriented, abnormal
Protruding-mouth skeletal myofibril assembly decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.