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ZFIN ID: ZDB-FIG-150218-7
Miyake et al., 2014 - Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain. PLoS One   9:e110836 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Effects of fgf16 on the development of glutamatergic neurons, GABAergic interneurons, and oligodendrocyte progenitor cells.

(A–D) The expression of ngn1 (A, B) and isl1 (C, D) in wild-type embryos (A, C) and fgf16 morphants (B, D) at 24 hpf. Lateral views with anterior to the left and dorsal to the top. (E–J) The expression of gad1 (E, F), olig2 (G, H), and slc17a6a (I, J) in wild-type embryos (E, G, I) and fgf16 morphants (F, H, J) at 28 hpf. Lateral views with anterior to the left and dorsal to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gad1b WT standard conditions Prim-5 GABAergic neuron ISH
Prim-5 nucleus of the tract of the postoptic commissure ISH
Prim-5 ventral telencephalon ISH
WT + MO1-fgf16 standard conditions Prim-5 GABAergic neuron ISH
Prim-5 nucleus of the tract of the postoptic commissure ISH
Prim-5 ventral telencephalon ISH
isl1 WT standard conditions Prim-5 diencephalon neuron ISH
Prim-5 epiphysis neuron ISH
Prim-5 telencephalon neuron ISH
WT + MO1-fgf16 standard conditions Prim-5 diencephalon neuron ISH
Prim-5 epiphysis neuron ISH
Prim-5 telencephalon neuron ISH
neurog1 WT standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO1-fgf16 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
olig2 WT standard conditions Prim-5 dorsal thalamus ISH
Prim-5 oligodendrocyte ISH
Prim-5 ventral telencephalon ISH
Prim-5 ventral thalamus ISH
WT + MO1-fgf16 standard conditions Prim-5 dorsal thalamus ISH
Prim-5 oligodendrocyte ISH
Prim-5 ventral telencephalon ISH
Prim-5 ventral thalamus ISH
slc17a6a WT standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
Prim-5 glutamatergic neuron ISH
WT + MO1-fgf16 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
Prim-5 glutamatergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-fgf16 standard conditions Prim-5 diencephalon neuron differentiation decreased process quality, abnormal
Prim-5 forebrain has fewer parts of type GABAergic neuron, abnormal
Prim-5 forebrain has fewer parts of type oligodendrocyte, abnormal
Prim-5 forebrain has normal numbers of parts of type glutamatergic neuron, normal
Prim-5 GABAergic neuron decreased amount, abnormal
Prim-5 GABAergic neuron differentiation disrupted, abnormal
Prim-5 oligodendrocyte differentiation disrupted, abnormal
Prim-5 ventral telencephalon neuron differentiation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One