FIGURE

Fig. 5

ID

ZDB-FIG-150129-13

Publication

Zhao et al., 2014 - Leptin and IL-6 Family Cytokines Synergize to Stimulate Müller Glia Reprogramming and Retina Regeneration

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Fig. 5

Leptin Signaling Is Necessary and Sufficient for Retina Regeneration

(A) RT-PCR analysis of mRNAs (from whole retina) encoding Leptin and Lepr at various times after retinal injury.

(B) In situ hybridization and BrdU immunofluorescence shows that lepa, lepb, and lepr RNAs are increased in BrdU⁺ MG-derived progenitors at the injury site. The asterisk marks the injury site (needle poke).

(C and D) MO-mediated knockdown of Lepr inhibits the generation of BrdU⁺ MG-derived progenitors at 4 dpi. p < 0.001, n = 4.

(E) qPCR shows that Lepr knockdown suppresses injury-dependent induction of reprogramming genes at 2 dpi. p < 0.01, n = 4.

(F) Intravitreal injection of recombinant human Leptin into the uninjured eye of 1016tuba1a:gfp fish stimulates GFP expression and BrdU incorporation in MG throughout the retina’s INL.

Error bars, SD. In (B) and (C), arrows point to MG-derived progenitors. Scale bars, 20 µm (B and C) and 50 µm (E). Primers are listed in Table S1. See also Figure S7.

Expression Data

Genes:	lepa lepb lepr
Fish:	WT
Condition:	physical alteration: retina
Anatomical Term:	Muller cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-lepr
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO2-lepr
Observed In:	Muller cell cell population proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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