header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150113-14
Muto et al., 2014 - Nipbl and Mediator Cooperatively Regulate Gene Expression to Control Limb Development. PLoS Genetics   10:e1004671 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 4 Nipbls are required for spatial patterns of hoxd expression in pectoral fin buds.

(A) Diagram of zebrafish hoxda cluster. (B, C) Expression of 3′-hoxd genes including hoxd3a and d4a (B) and 5′-hoxd genes including hoxd9a-d13a (C) was examined by ISH at 32 and 36 hpf to show both time-matched and stage-matched (nipbla/b-MO at 36 hpf and control at 32 hpf) comparisons. Dorsal views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hoxd3a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd4a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd9a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd10a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd11a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd12a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
hoxd13a AB control Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-15 pectoral fin bud embryonic pectoral fin morphogenesis decreased process quality, abnormal
Prim-25 pectoral fin bud embryonic pectoral fin morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.