ZFIN ID: ZDB-FIG-141023-10
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5

Fig. 1 Wnt signaling in the pharyngeal arches.

(A–H) In situ hybridization (ISH) and (I) quantitative, real-time PCR (qPCR) analysis of Wnt target gene expression; (A, C, E–H) lateral views, anterior to the left; (B, D) transverse sections through arch 2. (A, B) mycn mRNA is ventrally restricted (black arrowheads) in wild type (WT) embryos. Arches 1 and 2 are outlined by dotted lines. (C, D) GFP mRNA is ventrally restricted (arrowheads) in Tg(7xTCF-Xla.Siam:GFP)ia4 transgenics. (B, D) Both mycn (B) and 7xTCF;GFP (D) are expressed in ventral nc cells and pharyngeal endoderm, and excluded from pharyngeal ectoderm. (E–H) mycn and axin2 expression in controls (E, G), and dntcf3+ embryos at 26 hpf (F, H; heat shocked at 22 hpf). (I) qPCR analysis of axin2, lef1 and mycn expression in dntcf3+ embryos, normalized to nontransgenic, heat-shocked controls, with ef1alpha as an internal control. * P<0.05, ** P<0.001. Abbreviations: e, eye; D, dorsal arch; end, pharyngeal endoderm; hb, hindbrain; I, intermediate arch; mhb, mid-hindbrain boundary; nc, neural crest; pe, pharyngeal ectoderm; V, ventral arch. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
axin2 WT control Prim-5 eye ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
w26Tg/+ heat shock Prim-5 eye ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
GFP ia4Tg standard conditions Prim-5 pharyngeal arch neural crest ISH
Prim-5 Not Detected pharyngeal arch pharyngeal ectoderm ISH
Prim-5 pharyngeal arch pharyngeal endoderm ISH
Prim-5 pharyngeal arch ventral region ISH
mycn WT control Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ventral region ISH
WT standard conditions Prim-5 pharyngeal arch neural crest ISH
Prim-5 pharyngeal arch ventral region ISH
w26Tg/+ heat shock Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ventral region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.