ZFIN ID: ZDB-FIG-141023-11
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 2 Requirements for Wnt signaling in craniofacial cartilage development.

(A–C, F, G) Alcian blue stained cartilage at 96 hpf, dissected and flat mounted, ventral views, anterior to the left. (A) Wild-type control, (B) dkk1+/ heterozygote, (C) dkk1+/+ homozygote, (F) dntcf3+/ heterozygote, (G) dntcf3+/+ homozygote, heat shocked as described (see Results). Arrowheads indicate clefting of Meckel′s cartilage (Mc). (D) Histogram quantifying the frequency of Mc defects in dkk1+ embryos heat shocked at different stages from 16–36 hpf. (E) Histogram quantifying average Mc length, p<0.01. (H) Histogram quantifying the frequency of cartilage defects in arches 1 and 2 in dntcf3+ embryos heat shocked at different stages from 16–30 hpf. Abbreviations: Ch, ceratohyal; Hm, hyomandibular; Mc, Meckels; Pq, palatoquadrate; Sy, symplectic. Scale bar: 100 µm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg/+ heat shock Day 4 ceratohyal cartilage absent, abnormal
Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
w26Tg/w26Tg heat shock Day 4 ceratohyal cartilage absent, abnormal
Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
w32Tg/+ heat shock Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage split, abnormal
Day 4 pharyngeal arch cartilage decreased size, abnormal
w32Tg/w32Tg heat shock Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 hyomandibula decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 palatoquadrate cartilage decreased size, abnormal
Day 4 pharyngeal arch cartilage decreased size, abnormal
Day 4 symplectic decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.