FIGURE

Fig. 2

ID

ZDB-FIG-141016-47

Publication

Zdebik et al., 2013 - Epilepsy in kcnj10 Morphant Zebrafish Assessed with a Novel Method for Long-Term EEG Recordings

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Non-invasive recordings using a patch pipette on the surface of the optic tectum.

(A) Photo of the non-invasive EEG ZF recording set-up. To the left is a surface recording pipette, filled with 1 M NaCl. In the upper right is the reference electrode. Both are connected to the amplifier, as shown in the schematic in (B). The mounted fish is positioned on a microscope with which ZF viability can be monitored continuously. (C), long-term EEG recording of a kcnj10a morphant fish initially paralyzed in 20 mM D-tubocurarine. Fish were generally viable for over an hour, but paralysis appeared to wear off after 50 to 60 min. Movement artifacts ensued, associated with electrical activity and visible twitching (arrowhead). A 5 s period indicated by a box in C is represented above the trace in higher resolution.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-kcnj10a TL + MO2-kcnj10a
Knockdown Reagents:	MO1-kcnj10a MO2-kcnj10a
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One