FIGURE

Fig. S3

ID

ZDB-FIG-140811-27

Publication

Zaghloul et al., 2010 - Functional analyses of variants reveal a significant role for dominant negative and common alleles in oligogenic Bardet-Biedl syndrome

Other Figures

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Fig. S3

Quantification of gastrulation defects. (A) Movement of cells during gastrulation stages is defective in MO-injected and null or dominant negative mutant rescued embryos, whereas WT rescued embryos are similar to uninjected controls. (B-G) The extent of gastrulation movements was quantified by measurement of the width of the field of fluorescing cells, repeated in triplicate – error bars shown. Dominant negative mutations, such as BBS2 N70S (C) and BBS3 L170W (D), produce cell movement defects more severe than MO alone, while null mutations such as BBS10 Y197C (F) are similar to MO. Hypomorphic mutations, such as BBS1 M390R (B) and BBS12 A289P (G) are less severe than MO, but do not efficiently rescue the gastrulation movement phenotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-bbs1 WT + MO1-bbs10 WT + MO1-bbs12 WT + MO1-mkks WT + MO3-bbs2 WT + MO4-arl6
Knockdown Reagents:	MO1-bbs1 MO1-bbs10 MO1-bbs12 MO1-mkks MO3-bbs2 MO4-arl6
Observed In:	cell migration involved in gastrulation
Stage Range:	50%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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