FIGURE

Fig. 5

ID

ZDB-FIG-140808-5

Publication

Monteiro et al., 2014 - V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration

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Fig. 5

V-ATPase is required for blastema cell proliferation and expression of mkp3 and aldh1a2.

(A–D) Immunohistochemical detection of proliferating cells (H3P-positive) 24 h (A, C) and 48 h (B, D) after proximal amputation. (A, B) control fin; (C, D) V-ATPase knocked down fin. (E) Quantification of H3P-positive cells in the blastema. *statistical significant results (p<0.05). (F–U) In situ hybridization for mkp3 (F-M) and aldh1a2 (N-U), after proximal amputation (F, H, J, L; N, P, R, T) and distal amputation (G, I, K, M; O, Q, S, U): (F–G; N–O) control fin, 24 hpa; (H–I; P–Q) V-ATPase knocked down fin, 24 hpa; (J–K; R–S) control fin, 48 hpa; (L–M; T–U) V-ATPase knocked down fin, 48 hpa. Control fin: caudal fin from AB wild type fish, non-treated. V-ATPase knockdown procedure: atp6v1e1b vivo-MO delivered to the caudal fin of atp6v1e1b^hi577aTg/+ (AB) fish, 2 hpa. hpa: hours post amputation. For each panel, n = 3.

Expression Data

Genes:	aldh1a2 dusp6
Antibody:	Ab4-h3
Fish:	AB AB + MO1-atp6v1e1b atp6v1e1b^hi577aTg/+ + MO1-atp6v1e1b (AB) atp6v1e1b^hi577aTg/+ (AB)
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO1-atp6v1e1b
Anatomical Terms:	caudal fin caudal fin blastema
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp6v1e1b^hi577aTg/+ + MO1-atp6v1e1b (AB)
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO1-atp6v1e1b
Observed In:	fin regeneration regenerating fin cell population proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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