ZFIN ID: ZDB-FIG-140714-30
Nguyen-Chi et al., 2014 - Transient infection of the zebrafish notochord with E. coli induces chronic inflammation. Disease models & mechanisms   7:871-882 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec

Fig. 2

Behaviour of neutrophils and macrophages following notochord infection. (A) Diagram showing the regions of the larvae imaged in B and C (dashed boxes). (B) Tg(mpx:GFP) larvae that had been infected with crimson-expressing E. coli (magenta) in the notochord at 48 hpf. The general behaviour of neutrophils (green) was imaged using four-dimensional confocal microscopy, starting at 2 hpi for a duration of 3 hours (the time post-infection is shown in the top right corner). *Neutrophils loaded with bacteria, the arrowhead indicates the injection site. (C) Tg(mpeg1:mCherryF) larvae that had been infected with GFP-expressing E. coli (green) at 48 hpf in the notochord were imaged in the region represented in A to visualise the behaviour of macrophages (red) using four-dimensional confocal microscopy at 1 hpi for a duration of 3 hours and 20 minutes. Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP i114Tg bacterial treatment by injection: Escherichia coli K-12 Long-pec trunk neutrophil IFL
mCherry ump2Tg bacterial treatment by injection: Escherichia coli K-12 Long-pec trunk macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.