Fig. S2
Effect of PBS injection in the notochord on neutrophil recruitment. To check the effect of the injection in the notochord per se on neutrophil recruitment, PBS was injected in the notochord of Tg(mpx:GFP) larvae. No neutrophil was recruited to the notochord (visible with bright-field). To confirm and follow the injection in the notochord, PBS combined with Fluorecein-dextran (green) was injected in the notochord of Tg(mpx:GFP) larvae. Larvae were imaged at different time points following injection: 4, 24, 48 hpi and 4dpi and no recruited neutrophils (green) was detected. |