FIGURE

Fig. 6

ID

ZDB-FIG-140612-20

Publication

Bajard et al., 2014 - Wnt-regulated dynamics of positional information in zebrafish somitogenesis

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Fig. 6

Wnt target gene expression is reduced four to five somites after heat shock. (A-D,H-K) Dorsal view of flat-mounted PSM from hsdkk1::GFP (B,D,I,K) or WT siblings (A,C,H,J) heat shocked at five (A-D) or six (H-K) somites, fixed 1.5 (A,B), 1.75 (H,I) or 3 (C,D,J,K) hours phs and hybridized with spt (A-D) or msgn1 (H-K) riboprobes. (E,F,L,M) Average intensity profile for spt (E,F) and msgn1 (L,M) along the A/P axis. (G) Normalized logEC50 calculated from fitting plots of spt intensity profile at successive time points. (N) Maximum intensity of msgn1 normalized to hsWT dynamic range at successive time points. Mean ± s.d. ^*P<0.01, ^**P<0.001. Scale bars: 50 µm.

Expression Data

Genes:	msgn1 tbx16
Fish:	WT w32Tg (AB)
Condition:	heat shock
Anatomical Term:	segmental plate
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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