FIGURE

Fig. 4

ID

ZDB-FIG-150415-23

Publication

Bajard et al., 2014 - Wnt-regulated dynamics of positional information in zebrafish somitogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

A posterior gradient of Wnt activity is reduced <∼2 somites after heat shock. (A,B,E-H) Dorsal view of flat-mounted non-heat shocked (A,B), hsWT (E,G) or hsdkk1 (F,H) PSM at nine (G,H), ten (A,E,F) or 12 (B) somites. Embryos were fixed 30 minutes (E,F) or 1.5 hours (G,H) phs. (A) Fluorescence intensity of β-catenin staining after applying a nuclear mask, displayed using false color. (B) Whole-mount in situ hybridization with mespb (blue) and myoD (red) probes. (C,D,I,J) Nuclear intensity of β-catenin staining (C, percentage of maximum; D,I,J, grayscale values) along the PSM length for individual cells, for one embryo (C) or averaged within 1.5% bins for several embryos (D,I,J). Error bars reflect variation between embryos. The average mespb intensity profile is in purple in D. (K) Maximum intensity nuclear β-catenin normalized to hsWT dynamic range, with embryos fixed at successive time points. Mean ± s.d. ^*P<0.01, ^**P<0.001. Scale bars: 50 μm. See also supplementary material Figs S3, S4.

Expression Data

Genes:	mespba myod1
Antibody:	Ab1-ctnnb
Fish:	WT w32Tg (AB)
Condition:	heat shock
Anatomical Terms:	adaxial cell segmental plate anterior region segmental plate nucleus somite
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development