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ZFIN ID: ZDB-FIG-150415-23
Bajard et al., 2014 - Wnt-regulated dynamics of positional information in zebrafish somitogenesis. Development (Cambridge, England)   141:1381-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites

Fig. 4 A posterior gradient of Wnt activity is reduced <∼2 somites after heat shock. (A,B,E-H) Dorsal view of flat-mounted non-heat shocked (A,B), hsWT (E,G) or hsdkk1 (F,H) PSM at nine (G,H), ten (A,E,F) or 12 (B) somites. Embryos were fixed 30 minutes (E,F) or 1.5 hours (G,H) phs. (A) Fluorescence intensity of β-catenin staining after applying a nuclear mask, displayed using false color. (B) Whole-mount in situ hybridization with mespb (blue) and myoD (red) probes. (C,D,I,J) Nuclear intensity of β-catenin staining (C, percentage of maximum; D,I,J, grayscale values) along the PSM length for individual cells, for one embryo (C) or averaged within 1.5% bins for several embryos (D,I,J). Error bars reflect variation between embryos. The average mespb intensity profile is in purple in D. (K) Maximum intensity nuclear β-catenin normalized to hsWT dynamic range, with embryos fixed at successive time points. Mean ± s.d. *P<0.01, **P<0.001. Scale bars: 50 μm. See also supplementary material Figs S3, S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mespba WT control 10-13 somites segmental plate anterior region ISH
myod1 WT control 10-13 somites adaxial cell ISH
10-13 somites somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC WT control 5-9 somites segmental plate nucleus
IHC 10-13 somites segmental plate nucleus
IHC w32Tg(AB) heat shock 5-9 somites segmental plate nucleus
IHC 10-13 somites segmental plate nucleus
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development