FIGURE

Fig. 5

ID

ZDB-FIG-140515-6

Publication

Weber et al., 2014 - Mitotic Position and Morphology of Committed Precursor Cells in the Zebrafish Retina

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Fig. 5

Depletion of Specific Neuronal Subtypes Leads to Ectopic Basal Mitosis of Committed Precursors

(A) Control (left) and ath5 morphant embryo (MO; right) expressing Ptf1A:GFP (green) and stained for DAPI (blue). In the morphant, the prospective RGC layer is filled with Ptf1A+ cells.

(B) Ptf1A:GFP (green) embryo injected with ath5 MO and stained for pH3 (magenta) and DAPI (blue). Ptf1A+ division at very basal location (arrows).

(C) Percentage of cell divisions taking place beneath the IPL in control versus ath5 morphant embryos. Data are pooled from six embryos per time point.

(D) Cx55.5:rasGFP (green) ath5 morphant embryo stained for pH3 (magenta) and DAPI (blue). pH3+/Cx55.5+ cells are observed underneath the IPL (arrows).

(E) In ptf1A/ath5/p53 morphants (MO), an ectopic basal division close to the forming IPL occurs. pH3 is shown in magenta, Vsx1:GFP in green, and DAPI in blue.

(F) In ptf1A/ath5/p53 MO, ectopic Vsx1+ divisions are observed close to the ILM. pH3 is shown in magenta, Vsx1:GFP in green, and DAPI in blue.

Scale bars represent 10 μm. See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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