FIGURE

Fig. S2

ID

ZDB-FIG-140512-33

Publication

Alvers et al., 2014 - Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling

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Fig. S2

Gut tube shape and total cell number are similar in wildtype and mutant embryos. (A,B) In situ hybridization of WT and smo mutant embryos expressing foxa3 at 30 hpf. Scale bar: 200 μm. (C-D) WT and smo mutant embryo expressing TgBAC(cldn15la-GFP) at 48 hpf. Arrows indicate intestine. (E-F2) Cross section of WT and smo mutant guts at 72 hpf stained for BrdU to label proliferating cells. Scale bars: 20 μm. (G) Quantification of the diameter and perimeter of WT and smo mutant guts from transverse cross sections. Wt n=14, mutant n=18, diameter P>0.18, perimeter P>0.48. (H) Quantification of total cell number in WT and mutant guts. WT n=14, mutant n=18, P>0.30 (I) Quantification of the percent of BrdU positive cells in WT and smo mutant guts. WT n=14, mutant n=19, P>0.32.

Expression Data

Genes:	foxa3 GFP
Fish:	WT smo^s294/s294 pd1034Tg smo^s294/s294; pd1034Tg
Condition:	heat shock
Anatomical Term:	intestine
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^s294/s294
Observed In:	intestinal epithelial cell intestine
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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