ZFIN ID: ZDB-FIG-140512-31
Alvers et al., 2014 - Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Development (Cambridge, England)   141(5):1110-1119 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 6

Rab11 is abnormally localized in smos294 mutants. (A,A2) Confocal cross section of a Tg(hsp70l:gal4); Tg(UAS:rab11aS25N) embryo. Phalloidin (green). (B,B2) Confocal cross section of a Tg(hsp70l:rab11bS25N) embryo. Phalloidin (red) (C,D) Confocal cross sections of smos294 and wild-type clutchmates expressing Tg(hsp70l:GFP-RAB11a). Arrowheads point to abnormal enlarged Rab11 positive vesicles in smos294. Phalloidin (red) (E-H) Confocal cross section of wild-type and smos294 embryos expressing Tg(hsp70l:GFP-RAB11a) stained for the apical marker 4e8 (E,F) or cadherin (G,H). Arrowheads point to areas of colocalization. (I,J) Confocal cross sections of uninjected and RFP-Rab11fipa1-injected Tg(hsp70l:GFP-RAB11a) embryos. Arrowheads point to dispersed compartments. (K) Expression levels of Rab11 family members in the intestinal epithelium of smos294 mutants relative to wild-type clutchmates. Rab11fip1a P<0.011, n=3. All embryos are 72 hpf. Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP pd1031Tg heat shock Protruding-mouth intestinal epithelial cell lumenal side of membrane IFL
Protruding-mouth intestinal epithelium apical surface IFL
smos294/s294; pd1031Tg heat shock Protruding-mouth intestinal epithelial cell lumenal side of membrane IFL
Protruding-mouth intestinal epithelium apical surface IFL
mCherry kca4Tg; mw35Tg heat shock Protruding-mouth intestinal epithelial cell basolateral plasma membrane IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-4E8 IHC pd1031Tg heat shock Protruding-mouth intestinal epithelial cell lumenal side of membrane
IHC Protruding-mouth intestinal epithelium apical surface
IHC smos294/s294; pd1031Tg heat shock Protruding-mouth intestinal epithelial cell lumenal side of membrane
IHC Protruding-mouth intestinal epithelium apical surface
Ab2-pan-cadherin IHC pd1031Tg heat shock Protruding-mouth intestinal epithelial cell basolateral plasma membrane
IHC smos294/s294; pd1031Tg heat shock Protruding-mouth intestinal epithelial cell basolateral plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
smos294/s294; pd1031Tg standard conditions Protruding-mouth intestine anatomical space increased amount, abnormal
Protruding-mouth intestine epithelial tube morphogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development