FIGURE

Fig. 6

ID

ZDB-FIG-140512-31

Publication

Alvers et al., 2014 - Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling

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Fig. 6

Rab11 is abnormally localized in smo^s294 mutants. (A,A2) Confocal cross section of a Tg(hsp70l:gal4); Tg(UAS:rab11aS25N) embryo. Phalloidin (green). (B,B2) Confocal cross section of a Tg(hsp70l:rab11bS25N) embryo. Phalloidin (red) (C,D) Confocal cross sections of smo^s294 and wild-type clutchmates expressing Tg(hsp70l:GFP-RAB11a). Arrowheads point to abnormal enlarged Rab11 positive vesicles in smo^s294. Phalloidin (red) (E-H) Confocal cross section of wild-type and smo^s294 embryos expressing Tg(hsp70l:GFP-RAB11a) stained for the apical marker 4e8 (E,F) or cadherin (G,H). Arrowheads point to areas of colocalization. (I,J) Confocal cross sections of uninjected and RFP-Rab11fipa1-injected Tg(hsp70l:GFP-RAB11a) embryos. Arrowheads point to dispersed compartments. (K) Expression levels of Rab11 family members in the intestinal epithelium of smo^s294 mutants relative to wild-type clutchmates. Rab11fip1a P<0.011, n=3. All embryos are 72 hpf. Scale bars: 20 μm.

Expression Data

Genes:	GFP mCherry
Antibodies:	Ab-4E8 Ab2-pan-cadherin
Fish:	pd1031Tg kca4Tg; mw35Tg smo^s294/s294; pd1031Tg
Condition:	heat shock
Anatomical Terms:	intestinal epithelial cell basolateral plasma membrane intestinal epithelial cell lumenal side of membrane intestinal epithelium apical surface
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^s294/s294; pd1031Tg
Observed In:	intestine anatomical space intestine epithelial tube morphogenesis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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