Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-140311-43
Zhang et al., 2013 - Assessment of hematopoietic failure due to Rpl11 deficiency in a zebrafish model of Diamond-Blackfan anemia by deep sequencing. BMC Genomics   14:896 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

Rpl11 deficiency is required for hematopoietic defects through p53-independent pathways in zebrafish embryos. A-C, Rpl11 is required for HSC formation through p53-independent pathways. The expression of cmyb was significantly decreased in Rpl11 morphants and in Rpl11 and p53 double morphants at 48 hpf compared with that in control embryos. D-I, Rpl11 deficiency caused defective hematopoietic phenotypes in zebrafish embryos through p53-independent pathways. Hemoglobin staining of embryos individually injected with Rpl11 MO or Rps19 MO and coinjected with p53 MO were performed. D and G are controls, E and H are Rpl11 MO or Rps19 knockdown, and F and I are Rpl11 MO and Rps19 MO coinjected with p53 MO, respectively. Panel AC, lateral views; D-I, ventral views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myb WT standard conditions Long-pec hematopoietic stem cell ISH
WT + MO1-rpl11 standard conditions Long-pec hematopoietic stem cell ISH
WT + MO1-rpl11 + MO4-tp53 standard conditions Long-pec hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rpl11 standard conditions Long-pec hemopoiesis process quality, abnormal
WT + MO1-rpl11 + MO4-tp53 standard conditions Long-pec hemopoiesis process quality, abnormal
WT + MO1-rps19 standard conditions Long-pec hemopoiesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics