FIGURE

Fig. 1

ID

ZDB-FIG-140311-42

Publication

Zhang et al., 2013 - Assessment of hematopoietic failure due to Rpl11 deficiency in a zebrafish model of Diamond-Blackfan anemia by deep sequencing

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Fig. 1

Benzidine staining of Rpl11-deficient zebrafish embryos and the effectiveness of translational inhibition by Rpl11 MO. (A, B, C, D), O-staining shows markedly reduced numbers of hemoglobin-stained blood cells in Rpl11-knockdown embryos; (E, F), The Rpl11–egfp construct was assembled by inserting a partial sequence of Rpl11 cDNA (containing 60 bps from the 52 UTR) and the N-terminus of egfp into modified pEGFP-N1 (the ATG codon of EGFP was removed). The sequence of Rpl11 MO1 compliments 1–24 bp of Rpl11 cDNA. Embryos co-injected with 25 ng Rpl11–EGFP DNA and 0.5 ng control MO expressed EGFP, and Rpl11–EGFP expression was inhibited by co-injection with 0.5 ng Rpl11 MO; B, D, E, and F are the lateral view; A and C are the ventral view.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rpl11
Knockdown Reagent:	MO1-rpl11
Observed In:	hemopoiesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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