notch1a, notch1b and notch3 expression in radial glia and proliferating cells of the dorso-lateral telencephalic ventricular zone.
Confocal images showing localization of Notch receptors by FISH (white), radial glia labelled with S100β (red), and PCNA + proliferating cells (green); DAPI (blue) is used as nuclear counterstaining. Schematics in A indicate the cross-section levels through the telencephalon and the dorsal telencephalic area represented in the micrographs. A-B, notch1a and C-D, notch1b expressing cells are scattered throughout the dorso-lateral ventricular zone of the dT and localize with both PCNA +/S100β+ cells (arrowheads) and a subpopulation of PCNA -/S100β + cells (white arrows); yellow arrows indicate Notch receptor - /PCNA +/S100β- cells; unfilled yellow arrowheads indicate Notch receptor - /PCNA +/S100β- cells. E-F, notch3 expressing cells localize to a great extent with S100β, including both PCNA (white arrows) and PCNA (white arrowheads) cells; unfilled yellow arrowheads indicate notch3 -/PCNA + cells. G, Quantification on the co-localization of Notch receptor by FISH with PCNA and S100β, for the Dl region; schematics on the left indicate the cross-section levels through the telencephalon, along the rostro-caudal axis, and examples of Dl areas used for marker co-localization analysis; all ventricularly located cells of the indicated area were counted; cells were distinguished based on their glia character (S100β +), notch expression and proliferative status (PCNA +). n = 9 (fish), 4–6 tissue sections per fish, at different rostro-caudal levels; total number of cells counted = 6433. Values represented as mean percentage ± SEM. Significance levels: p<0.001(***),p<0.01(**) ,p<0.05(*) . Abbreviations: Dl, dorso-lateral telencephalic area; dT, dorsal telencephalic area; vT, ventral telencephalic area; Scale bars = 50 μm in C (applies to A) and in E; 10 μm in B, D and F.
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