Fig. S4

notch3 expression in glia and proliferating cells of the adult zebrafish hypothalamus. Confocal images showing localization of notch3 mRNA by FISH (white), radial glia labelled with S100β (red), and PCNA + proliferating cells (green). Cross-sections at the indicated levels through the diencephalon; hypothalamic area shown in the micrographs is indicated in the cross-section schematics. A, B, notch3 is expressed in most PCNA+ /100β- cells (filled white arrowheads) and in PCNA +/S100β - cells (unfilled white arrowheads) of the Hv; unfilled yellow arrowheads indicate notch3 - /PCNA +/S100β cells. A–F, notch3 localizes with most S100β cells of Hd and Hc, PCNA + (filled white arrowheads) or PCNA (filled white arrows); filled yellow arrowhead indicates a notch3 -/PCNA +/S100β + cell. Notice the notch3 expression in the S100β + cellular processes in Hc (in E). Asterisk indicates a S100β group of cells in Hd that is negative for notch3. Abbreviations: Hc, caudal zone of the periventricular hypothalamus; Hd, dorsal zone of the periventricular hypothalamus; Hv, ventral zone of the periventricular hypothalamus: PR, posterior recess of the diencephalic ventricle. Scale bar = 100 μm in A and D, 20 μm in B, B , C, C , E, F, F .