FIGURE

Fig. 2

ID

ZDB-FIG-131218-55

Publication

Cavodeassi et al., 2013 - Eph/Ephrin signalling maintains eye field segregation from adjacent neural plate territories during forebrain morphogenesis

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Fig. 2

Partial abrogation of Eph/Ephrin activity interferes with optic vesicle evagination. Whole-mount in situ hybridisation in 10-12 ss wild-type zebrafish embryo (A), embryos injected with Eph/Ephrin morpholinos (B,C) or mRNA (D,F), and efnb2a mutant (E). The optic vesicles are labelled by expression of mab21/2. Arrows (B-F) indicate the presence of eye fated cells (labelled by mab21/2) embedded within the forebrain. All panels show dorsal views with anterior to the left. The phenotype shown in C is representative of 61% of the embryos analysed; the remaining 39% are not wild type but show a milder phenotype than that illustrated. The phenotype shown in F is representative for EphA4a and EphB4a misexpressions (epha4a: 70%, n=56; ephb4a: 100%, n=25). mb, midbrain; ov, optic vesicle. Scale bars: 50 μm.

Expression Data

Gene:	mab21l2
Fish:	WT efnb2a^{hu3393/hu3393} WT + MO1-efnb1 + MO1-efnb2a WT + MO1-efnb1 + MO1-efnb2a + MO1-epha4a
Knockdown Reagents:	MO1-efnb1 MO1-efnb2a MO1-epha4a
Anatomical Terms:	forebrain immature eye midbrain
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	efnb2a^{hu3393/hu3393} WT + MO1-efnb1 + MO1-efnb2a WT + MO1-efnb1 + MO1-efnb2a + MO1-epha4a
Knockdown Reagents:	MO1-efnb1 MO1-efnb2a MO1-epha4a
Observed In:	optic vesicle morphogenesis
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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