ZFIN ID: ZDB-FIG-131213-17
Geng et al., 2013 - Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene. Development (Cambridge, England)   40(21):4362-4374 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 7 Comparison of expression of gpr126 and sox10 in wild-type embryos, and expression of gpr126 in colourless (sox10-/-) mutant embryos. (A-D) Expression of gpr126 and sox10 in wild-type embryos at 24 hpf (A,B) and 48 hpf (C,D). Both genes are expressed in the otic vesicle (arrowhead), post-otic region (arrow), olfactory epithelium (nose, n) and head chondrocytes (asterisks). (E-G) Dorsal views of flat-mounted 24 hpf embryos: post-otic expression of gpr126 in the wild type (E) is lost in the cls mutant (F). Comparison with foxd3 expression identifies these cells as neural crest (G). (H,I) Dorsal view of the post-otic region showing foxd3-expressing Schwann cells extending posteriorly (H, arrow) and expression of gpr126 in the same location (I, arrow). (J,K) gpr126 expression in heart (h) and posterior mesoderm (m) persists in cls mutants, whereas neural crest expression is lost (arrows). (L,M) Expression of gpr126 is reduced in the cls mutant ear at 48 hpf, with weak expression in rudimentary projections (M). (N,O) DIC images of live ears at 96 hpf, showing a representative cls mutant ear (O). Note the small overall size and rudimentary unfused canal projections (arrowheads). Abbreviations: h, heart; m, posterior mesoderm, n, nose (olfactory epithelium); nc, neural crest; ov, otic vesicle; sib, phenotypically wild-type sibling embryo. Scale bars: in D, 100 μm for A-D; in G, 50 μm for to E-G; in I, 50 μm for H,I; in K, 100 μm for J,K; in L, 50 μm for L-O.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
adgrg6 WT standard conditions Prim-5 heart tube ISH
Prim-5 immature Schwann cell ISH
Prim-5 mesoderm posterior region ISH
Prim-5 neural crest ISH
Prim-5 otic vesicle ISH
Prim-5 otic vesicle posterior_to ISH
Prim-5 primitive olfactory epithelium ISH
Long-pec head chondrocyte ISH
Long-pec otic vesicle ISH
Long-pec otic vesicle protrusion ISH
sox10t3/t3 standard conditions Prim-5 heart tube ISH
Prim-5 Not Detected immature Schwann cell ISH
Prim-5 mesoderm posterior region ISH
Prim-5 Not Detected neural crest ISH
Prim-5 Not Detected otic vesicle posterior_to ISH
Long-pec otic vesicle protrusion ISH
foxd3 WT standard conditions Prim-5 immature Schwann cell ISH
Prim-5 neural crest ISH
sox10 WT standard conditions Prim-5 neural crest ISH
Prim-5 otic vesicle ISH
Prim-5 otic vesicle posterior_to ISH
Prim-5 primitive olfactory epithelium ISH
Long-pec head chondrocyte ISH
Long-pec otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox10t3/t3 standard conditions Day 4 semicircular canal disorganized, abnormal
Day 4 semicircular canal malformed, abnormal
Day 4 semicircular canal fusion arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development